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Computational Analysis of Specific IgE Epitopes Responsible for Allergy to Fish

[ Vol. 14 , Issue. 2 ]


Mohsen Mohammadi, Reza Falak*, Reza Zolfaghari Emameh, Soheila June Maleki and Gholam Ali Kardar   Pages 130 - 136 ( 7 )


Background: Fish is one of the most common causes of IgE-mediated adverse reactions to food. Several allergens have been identified and characterized from different fish species, among them, parvalbumin, as a highly conserved protein, is the most common causative agent of fish-borne allergies. Although most fish-allergic patients are sensitive to various species, some patients only show hypersensitivity to certain species such as salmonids. In this study, we computationally identified and mapped mono-sensitivity-causing Epitopes of salmonid fish and their cross-reactive Epitopes using in silico methods.

Methods: Amino acid sequences of parvalbumins were retrieved from NCBI, and following alignment, the phylogenic tree was drawn and potential Epitopes were determined and their physicochemical properties analyzed.

Results: We found that fish-allergic patients are mostly sensitized to beta-1 isoform of parvalbumin and its epitope C region (65-109) in salmonids is probably the causative agent of monosensitivity, while in beta-2 isoforms it may justify cross-reactivity of parvalbumins.

Conclusion: Surely, any progresses in biochemical, immunological, and molecular mechanism of allergic reactions to fish allergens can improve accurate diagnosis of fish allergy and its' prevention and treatment in the future.


Calcium-binding proteins, mono-sensitivity, cross-reactivity, parvalbumin, allergy, IgE.


Persian Gulf Marine Biotechnology Research Center, Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Immunology Research Center, Iran University of Medical Sciences, Tehran, Department of Energy and Environmental Biotechnology, Division of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, US Department of Agriculture, Agricultural Research Service, Southern Regional Research Center, New Orleans, LA, Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran

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